FastQCFastQC Report
Fri 10 Feb 2017
ERR1147451.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147451.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2926322
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT298151.0188557513493048No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183270.6262810449431061No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147070.5025762715107907No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47430.1620805912678099No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43330.14806982963597307No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT33370.11403393064741338No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA31290.10692603206345712No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA206050.054.3260461
GGTATCA111150.052.5511741
AACGCAG255050.043.746956
ATCAACG274550.040.6718983
TCAACGC275450.040.5286144
CAACGCA280400.039.897995
TATCAAC282250.039.7316362
ACGCAGA297400.037.4973537
CGCAGAG300600.037.117968
GCAGAGT319100.035.1151359
CATGGGT84850.033.295234
GTACATG271250.032.026421
ATGGGTG44400.031.7472155
TACATGG273100.031.7392652
ACATGGG272550.031.2352543
AGAGTAC314900.028.70854610-11
CATGGGG126700.028.071454
AGTACTT162650.027.7907212-13
GAGTACT157300.027.69613612-13
CAGAGTA318550.026.76457610-11
Produced by FastQC (version 0.11.5)