FastQCFastQC Report
Fri 10 Feb 2017
ERR1147448.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147448.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1709592
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144180.8433591172630662No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92720.5423516254170585No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71590.41875488420629015No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23220.1358218803082841No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21960.12845170075667176No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA103250.055.180981
GGTATCA54550.055.1142771
AACGCAG133500.042.3726436
TATCAAC140850.040.2119672
TCAACGC140300.040.1493574
ATCAACG140850.039.998433
CAACGCA143600.039.2267075
ACGCAGA154650.036.6546677
CGCAGAG157850.035.8362248
GCAGAGT167650.033.7768949
GTACATG143050.031.1717741
TACATGG146400.030.4786282
ACATGGG146950.029.9579413
AGAGTAC164100.028.85298710-11
CATGGGT41500.027.2327694
AGTACTT83450.026.83644312-13
ATGGGTG22450.026.760325
GAGTACT80950.026.74673812-13
CATGGGG73350.026.5175824
TAGATCG900.00954422526.4364875
Produced by FastQC (version 0.11.5)