FastQCFastQC Report
Fri 10 Feb 2017
ERR1147447.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147447.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1816297
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184891.0179502581350957No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113690.6259438847281034No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92750.5106543698525077No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31790.17502644116022875No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24850.13681683116803034No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT23500.12938412605427416No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG23180.12762229965693936No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA22070.12151096434118429No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA21050.11589514269967963No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT20910.11512434365084565No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC19830.1091781795598407No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC19290.10620509751433824No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC18900.10405787159258645No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA149400.046.5320661
AACGCAG167650.041.36726
GGTATCA89900.040.5183561
ATCAACG190950.036.4159663
TCAACGC192150.036.15464
CAACGCA195550.035.586825
ACGCAGA195500.035.5046467
CGCAGAG197450.035.093768
TATCAAC203800.034.208372
GCAGAGT207900.033.444229
GTACATG170450.029.8907551
TACATGG173950.029.340792
ACATGGG173750.029.1341083
CATGGGG85250.027.9078734
AGAGTAC205000.027.20060210-11
ATGGGAG16450.026.3947815
GAGTACT108200.026.1662112-13
CAGAGTA207750.025.49493810-11
AGTACTT111000.025.45257812-13
CATGGGT37700.024.7696234
Produced by FastQC (version 0.11.5)