FastQCFastQC Report
Fri 10 Feb 2017
ERR1147445.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147445.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2403300
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT383141.594224607830899No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228150.9493196854325302No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207920.8651437606624225No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66720.27761827487205093No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53870.2241501269088337No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA45110.18770024549577663No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35390.14725585653060375No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31010.12903091582407522No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA29930.12453709482794491No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28010.11654807972371323No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA28010.11654807972371323No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA27230.1133025423376191No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA307950.044.123451
AACGCAG339300.039.8797156
GGTATCA190300.037.3430061
ATCAACG396350.034.2623943
TATCAAC398100.034.1580282
CGCAGAG396050.034.1353158
ACGCAGA395900.034.103187
TCAACGC400550.033.915164
CAACGCA407200.033.3320775
GCGTATC2005.2750693E-1132.7345541
GCAGAGT416800.032.507279
CATGGGT56400.029.844334
GTACATG328300.029.6771051
ACATGGG330000.029.1825713
TACATGG334900.029.1120342
CGTATCA2252.0736479E-1029.0816422
ATGGGTG32850.028.7883195
CATGGGG158850.028.2692494
AGAGTAC413700.027.0144310-11
AGTACTT230300.026.43311912-13
Produced by FastQC (version 0.11.5)