FastQCFastQC Report
Fri 10 Feb 2017
ERR1147444.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147444.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2895190
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT371981.2848206853436217No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223440.7717628204021152No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192180.6637906320483284No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59900.206894884273571No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58290.20133393663282892No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32920.11370583623181899No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29210.10089147862489163No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA242400.055.5940931
GGTATCA126050.054.210561
AACGCAG303550.044.2426266
TATCAAC326550.041.2428742
ATCAACG326300.041.1629563
TCAACGC329800.040.7752954
CAACGCA336450.040.004725
ACGCAGA351100.038.2677237
CGCAGAG353900.037.9481438
GCAGAGT373200.036.033479
GTACATG300800.032.571431
CATGGGT91550.032.353274
TACATGG304500.032.117132
ACATGGG304100.031.610013
ATGGGTG49700.031.2342685
CATGGGG148850.029.7683414
AGAGTAC368850.029.75046710-11
TATCACG2001.869921E-929.7435112
ATGGGGG61250.029.7140965
AGTACTT203450.028.00625412-13
Produced by FastQC (version 0.11.5)