FastQCFastQC Report
Fri 10 Feb 2017
ERR1147443.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147443.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1224373
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124221.0145601054580589No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84720.6919460001159777No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60890.4973157689690968No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19190.15673328307631743No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC17560.14342034657739103No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14950.12210331328769909No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57250.052.2991141
GTATCAA97500.047.620271
AACGCAG115600.040.1910136
TATCACG957.4822474E-637.571922
TATCAAC124050.037.5012472
GCCTAAT1751.0913936E-1137.4159241
GGGTTAG5950.037.0157551
ATCAACG124900.037.007893
TCAACGC126050.036.5758634
CAACGCA129050.035.7255945
ACGCAGA138000.033.7965777
CGCAGAG139050.033.541378
GTTAGAG6450.032.2807853
GTACATG120250.032.0768551
GCAGAGT145750.032.040329
GTAAGTA16250.031.8494262
ATATGTA8300.031.55561
ATGGGGG37150.031.5459165
TACATGG122350.031.4097352
TGTAAGT16350.030.9458921
Produced by FastQC (version 0.11.5)