FastQCFastQC Report
Fri 10 Feb 2017
ERR1147442.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147442.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2468872
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT248411.006167998989012No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147410.5970742914172951No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121180.49083144043109567No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40750.165055134490569No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31070.1258469454876559No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA199550.046.620961
GGTATCA119400.041.9242821
AACGCAG238000.038.8075756
TCAACGC258700.035.9092754
ATCAACG258750.035.8592723
TATCAAC261600.035.651212
CAACGCA261600.035.4657255
ACGCAGA273500.033.792147
CGCAGAG276950.033.328248
GCAGAGT292950.031.5891639
ATGGGGG46750.030.6589625
GTACATG250000.029.2607751
TACATGG253200.028.7765272
ACATGGG250950.028.5125223
CATGGGG120800.028.358134
CATGGGT48350.028.1683254
GAGTACT133000.027.3666612-13
AGTACTT136550.026.43741812-13
AGAGTAC285600.026.18616110-11
GTAAGTA18350.025.2827642
Produced by FastQC (version 0.11.5)