FastQCFastQC Report
Fri 10 Feb 2017
ERR1147439.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147439.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2044902
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152110.7438498275222969No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90930.4446667859877882No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74080.36226674921340973No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25370.12406462510183862No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21430.10479719810533707No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61050.052.7610441
GTATCAA108550.050.7905581
AACGCAG134100.040.8081136
TCAACGC144750.037.764564
ATCAACG145500.037.5726473
CAACGCA147500.037.1008035
TATCAAC149350.036.6855472
ACGCAGA154300.035.3886687
CGCAGAG158550.034.440068
GCAGAGT168050.032.5639279
TATCACG1152.7683398E-531.038022
GTACATG138850.030.530641
TACATGG141050.029.860752
ACATGGG143500.029.059323
AGAGTAC163500.027.90391210-11
CATGGGT29550.026.5707554
CAGAGTA165550.026.2828610-11
GAGTACT86200.026.18737612-13
CATGGGG77800.025.3831754
AGTACTT89950.025.02950112-13
Produced by FastQC (version 0.11.5)