FastQCFastQC Report
Fri 10 Feb 2017
ERR1147438.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147438.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3206338
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT279650.8721787908823088No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167730.5231201451624875No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142590.4447129404323562No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46030.14355941263834318No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35450.11056226760871748No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA195100.051.2570341
GGTATCA107850.047.300111
AACGCAG228350.043.5756726
TCAACGC252250.039.3055234
ATCAACG254000.039.0143433
CAACGCA256700.038.6473165
TATCAAC259200.038.622972
ACGCAGA267450.037.182867
CGCAGAG273100.036.3700528
GCAGAGT292300.034.1641859
GTACATG232350.031.6648351
TACATGG237750.030.9989762
ACATGGG242150.030.0417373
CATGGGG127350.029.4699634
AGAGTAC283600.028.1551110-11
TATAACG3750.026.9658582
CATGGGT48050.026.2416784
AGTACTT162950.026.15236712-13
CAGAGTA290250.026.10636110-11
GAGTACT159150.026.01068312-13
Produced by FastQC (version 0.11.5)