FastQCFastQC Report
Fri 10 Feb 2017
ERR1147437.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147437.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3330463
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234950.7054574694269236No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142000.4263671447483428No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129890.39000583402367783No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40760.12238538605593276No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA168200.050.957861
GGTATCA95150.050.2946621
AACGCAG210850.040.5093736
TCAACGC224450.038.00184
ATCAACG226200.037.6837653
TATCAAC228750.037.5237272
CAACGCA230100.037.0686845
ACGCAGA244950.034.8699767
CGCAGAG249500.034.2817548
GCAGAGT270650.031.6687269
GTACATG222150.030.9196221
TACATGG227900.030.1205022
ACATGGG225300.029.6232283
AGAGTAC261200.027.38334810-11
CATGGGG124000.027.2459094
CATGGGT43450.026.0098884
AGTACTT150350.025.65554212-13
CAGAGTA265950.025.02676810-11
GAGTACT144900.024.69118112-13
ATGGGGG57600.024.4737515
Produced by FastQC (version 0.11.5)