FastQCFastQC Report
Fri 10 Feb 2017
ERR1147436.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147436.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3383354
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT341741.0100627956755337No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205890.608538154742306No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181600.5367454898305054No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59630.176245228846878No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54290.1604620740247695No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA234400.051.030681
GGTATCA129450.046.1785961
AACGCAG285250.041.683676
ATCAACG312650.038.1475683
TCAACGC314850.037.840434
TATCAAC318800.037.525282
CAACGCA321650.037.0219545
ACGCAGA336050.035.4355357
CGCAGAG337450.035.2532658
GCAGAGT360200.033.191839
GTACATG286850.033.1481361
TACATGG291050.032.64132
ACATGGG292150.031.845043
CATGGGG150950.031.247764
CATGGGT59850.031.2064484
ATGGGGG60150.029.0730445
AGAGTAC353100.028.03073110-11
ATGGGTG38150.026.505335
AGTACTT203700.026.03209712-13
TATAACG3200.026.0260492
Produced by FastQC (version 0.11.5)