FastQCFastQC Report
Fri 10 Feb 2017
ERR1147435.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147435.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2360684
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT273521.1586472395288823No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162660.689037584022258No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145460.616177345210117No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45870.19430809036702923No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42950.18193879401054946No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25620.10852786734692149No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA192550.049.3701551
GGTATCA111050.044.0612031
AACGCAG226800.041.491866
TATAACG2000.038.6686332
ATCAACG255350.037.065563
TATCAAC257100.036.8603252
TCAACGC257050.036.7247434
CAACGCA262450.035.9464535
ACGCAGA266750.035.27787
CGCAGAG268900.035.0178578
GCAGAGT284150.033.222239
GTACATG227250.032.7946931
TACATGG230800.032.168012
ACATGGG232100.031.7052253
CATGGGT48600.031.210694
CATGGGG124100.030.053514
ATGGGGG43550.029.3663355
ATGGGTG31450.028.7489785
AGAGTAC281100.028.6204110-11
ATGGGAG23050.027.0967885
Produced by FastQC (version 0.11.5)