FastQCFastQC Report
Fri 10 Feb 2017
ERR1147434.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147434.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3511051
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT385711.098559946864913No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219340.6247132268941693No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196060.5584082942685822No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61080.1739650036413598No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57080.16257240353387062No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA254900.054.9401051
GGTATCA134600.052.6852071
AACGCAG315000.044.3373956
ATCAACG338300.041.3077663
TCAACGC341550.040.9082954
TATCAAC346550.040.429122
CAACGCA348700.040.086545
ACGCAGA364150.038.3040927
CGCAGAG368050.037.898218
GCAGAGT394900.035.4720579
GTACATG327400.032.9716841
TACATGG333300.032.3081782
ACATGGG331800.032.1121863
TATCACG2450.031.567962
CATGGGT110100.030.5781924
CATGGGG157000.029.8923444
ATGGGTG59950.029.2695125
AGAGTAC388700.028.96036310-11
ATGGGGG61950.028.5166025
CAGAGTA393100.026.9036610-11
Produced by FastQC (version 0.11.5)