FastQCFastQC Report
Fri 10 Feb 2017
ERR1147431.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147431.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2577695
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT268461.0414730990283956No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166950.6476716601459831No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137620.5338878338981145No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45720.1773677646114067No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41130.15956115832167886No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA102050.052.087991
GTATCAA182950.051.700011
AACGCAG223050.042.3449756
ATCAACG242600.038.984653
TCAACGC242400.038.9401784
TATCAAC246600.038.4254042
CAACGCA246650.038.2692035
ACGCAGA259900.036.295317
CGCAGAG263200.035.840248
GCAGAGT282050.033.55049
GTACATG223100.030.949781
TACATGG227350.030.3500082
ACATGGG227400.029.610333
CATGGGT75750.029.2088154
AGAGTAC277550.027.96551910-11
CATGGGG101300.027.595744
AGTACTT157050.026.32088512-13
CAGAGTA282450.026.20637510-11
GAGTACT152550.026.20056512-13
TATAACG2507.039489E-1026.1726932
Produced by FastQC (version 0.11.5)