FastQCFastQC Report
Fri 10 Feb 2017
ERR1147430.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147430.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2746073
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68250.24853672863030227No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43140.15709706187708775No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34080.12410449394462564No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA89750.053.7293781
GGTATCA48350.050.4834021
AACGCAG115950.040.7828836
TCAACGC120150.039.3077554
CAACGCA122900.038.379815
ATCAACG123850.038.1856423
TATCAAC128750.037.0564652
ACGCAGA139500.033.812757
GTACATG174950.033.6205061
CGCAGAG145750.032.4852378
TACATGG180000.032.289322
ACATGGG179400.032.065133
ATGGGGG54500.031.3232565
CATGGGG104250.031.2099294
GCAGAGT158900.029.9466069
TGGGGGG43500.028.7151816
ATATGTA13650.027.476991
AGAGTAC150550.025.89843210-11
TATGTAA16150.024.6796592
CAGAGTA156700.022.98405610-11
Produced by FastQC (version 0.11.5)