FastQCFastQC Report
Fri 10 Feb 2017
ERR1147429.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147429.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5185965
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT302150.5826302337173506No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159800.30813937232511207No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159380.30732949412500854No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56150.10827300222812919No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA197400.055.698971
GGTATCA104200.051.0736921
AACGCAG246550.044.221036
TCAACGC258350.042.155214
ATCAACG261500.041.6286773
CAACGCA264600.041.1819575
TATCAAC284900.038.5238532
ACGCAGA291450.037.4492727
CGCAGAG301900.036.2318048
GCAGAGT325300.033.7900859
GTACATG269200.030.8479711
TACATGG281400.029.595782
ACATGGG280200.029.148463
AGAGTAC309000.028.16159210-11
CATGGGG150550.027.1818524
CAGAGTA320650.025.45037810-11
GAGTACT169450.025.29087312-13
AGTACTT177900.025.02575712-13
GTATACG2201.5336627E-724.3527091
CATGGGT66700.023.6315084
Produced by FastQC (version 0.11.5)