FastQCFastQC Report
Fri 10 Feb 2017
ERR1147426.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147426.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2012967
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160150.7955917806898971No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97280.4832667400906225No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79740.3961316802510921No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28570.14192979815367068No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25970.1290135407088144No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA122400.049.851581
GGTATCA69750.046.8559341
AACGCAG144350.041.9469646
TCAACGC158750.038.1794784
ATCAACG159100.038.137613
CAACGCA161950.037.3883555
TATCAAC166500.036.7311672
ACGCAGA169100.035.8074767
CGCAGAG172950.035.0791558
GCAGAGT183600.033.109139
GTACATG159900.032.4269521
TACATGG162800.031.9018382
ACATGGG163850.031.4045893
GCGTATC1152.7554623E-531.0591741
CATGGGG78650.029.7966234
CATGGGT42150.029.3518374
ATGGGGG29050.027.8459685
AGAGTAC178850.027.20407110-11
TATCACG1107.759649E-427.0416492
ATGGGTG24750.026.4354695
Produced by FastQC (version 0.11.5)