FastQCFastQC Report
Fri 10 Feb 2017
ERR1147424.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147424.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2544957
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216390.8502697688015947No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132390.5202052529767693No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110360.4336419043622348No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36790.14456039925232528No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA177400.045.564911
GGTATCA106800.041.4096831
AACGCAG196300.040.994016
ACGCAGA227400.035.4659967
ATCAACG227200.035.316053
TCAACGC228400.035.1544764
CGCAGAG230050.035.005758
CAACGCA233000.034.434915
TATCAAC235250.034.311192
GCAGAGT245450.032.979049
GTACATG201150.032.6096341
TACATGG204850.031.8534342
ACATGGG204100.031.6195163
CATGGGG109150.031.2774314
CATGGGT42350.031.1775864
AGAGTAC238800.027.62104610-11
ATGGGGG42950.026.172285
CAGAGTA245000.025.62330210-11
GAGTACT129250.025.0789212-13
AGTACTT134150.024.6949112-13
Produced by FastQC (version 0.11.5)