FastQCFastQC Report
Fri 10 Feb 2017
ERR1147423.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147423.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2450110
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208620.8514719747276652No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126980.5182624453595961No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106000.43263363685712064No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35720.14578937272204104No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32450.1324430331699393No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA149550.050.1217651
GGTATCA84550.047.5310251
AACGCAG180600.041.3667146
ATCAACG196250.038.0135083
TCAACGC197550.037.78734
CAACGCA201300.037.0242655
TATCAAC207500.036.0958862
ACGCAGA211250.035.3648727
CGCAGAG214200.034.9333578
GCAGAGT229100.032.8690959
GTACATG187900.031.748851
TACATGG191850.030.853992
ACATGGG192950.030.1231143
CATGGGG99950.029.219844
AGAGTAC224650.027.25832210-11
ATGGGGG37750.026.943775
CAGAGTA228050.025.2478510-11
AGTACTT122200.025.18942812-13
GAGTACT118950.024.85255812-13
ATGGGAG20050.024.6231085
Produced by FastQC (version 0.11.5)