FastQCFastQC Report
Fri 10 Feb 2017
ERR1147422.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147422.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3857496
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT359670.9323924120724947No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206180.5344918050465898No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182350.4727159794851375No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59050.15307857739839523No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47450.12300725652081039No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA239500.050.2332761
GGTATCA137100.048.3702661
AACGCAG290800.041.0488246
ATCAACG318350.037.6134343
TCAACGC318950.037.556464
TATCAAC323300.037.241382
CAACGCA326250.036.7161145
ACGCAGA339000.035.2825627
CGCAGAG345300.034.6043858
GCAGAGT367450.032.5669829
GTACATG293250.031.1399631
TACATGG300400.030.2978952
ACATGGG298600.029.9813213
CATGGGG141050.027.7881814
AGAGTAC358950.027.74590910-11
CATGGGT56700.026.3292474
CAGAGTA365050.025.43304310-11
GAGTACT200100.024.75971812-13
AGTACTT208250.024.6903812-13
ATGGGAG46300.024.5357955
Produced by FastQC (version 0.11.5)