FastQCFastQC Report
Fri 10 Feb 2017
ERR1147421.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147421.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1790564
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153100.8550378539946073No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87680.48967811259469085No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78100.43617541735453186No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26610.14861239251989874No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18870.10538578905864297No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA127150.044.747381
AACGCAG139650.040.4177676
GGTATCA77800.038.5546721
GCGTATC1001.0606909E-535.708971
ATCAACG163750.034.5791933
GTACATG154400.034.4986271
TCAACGC164850.034.3474924
ACGCAGA165700.034.099517
TATCAAC167500.033.9470672
TACATGG158150.033.7727432
CAACGCA167650.033.702895
ACATGGG156000.033.590043
CGCAGAG168700.033.493128
CATGGGT30150.032.5494544
CATGGGG78700.031.514354
TATCACG1701.2656528E-831.4885582
GCAGAGT181450.031.1396479
ATGGGGG24300.028.3921815
AGAGTAC178450.025.99711210-11
GGGGTGA27850.025.6273147
Produced by FastQC (version 0.11.5)