FastQCFastQC Report
Fri 10 Feb 2017
ERR1147418.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147418.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2695740
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT333671.2377677372446898No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186010.6900146156528448No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173580.6439048276169067No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56920.21114795937293654No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47600.17657489223738193No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27040.10030640937182368No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA224050.050.62131
GGTATCA126500.048.382221
AACGCAG257800.043.654256
ATCAACG293300.038.4363253
TATCAAC294850.038.296222
TCAACGC294200.038.2935524
CAACGCA299600.037.5834965
ACGCAGA300800.037.433567
CGCAGAG303800.037.0639088
ATGGGGG58950.035.3160865
GCAGAGT320650.035.2460679
GTACATG262850.034.3153081
CATGGGG146000.033.896814
TACATGG268850.033.5688442
ACATGGG266850.033.3287243
CATGGGT46950.030.4063264
ATATGTA16500.029.587831
AGAGTAC317500.029.36649910-11
AGTACTT181100.027.47488212-13
TGGGGGG52300.027.409666
Produced by FastQC (version 0.11.5)