FastQCFastQC Report
Fri 10 Feb 2017
ERR1147416.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147416.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1915445
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT235461.2292704828381917No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146920.7670280274296573No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117590.613904340766767No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39410.20574853363056625No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31860.16633210559426137No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC20930.1092696475231604No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA167650.048.9094051
GGTATCA97750.047.8205831
AACGCAG195600.041.5704966
ATCAACG219550.037.1498953
TATCAAC220100.037.1381532
TCAACGC222350.036.6763344
TATCACG1156.668197E-736.2121052
CAACGCA225700.036.0792475
ACGCAGA228800.035.564417
CGCAGAG231100.035.2104578
GTACATG203900.033.584791
GCAGAGT243600.033.5013549
TACATGG207400.032.9583052
ACATGGG208250.032.2524383
CATGGGG115600.030.9244674
ATGGGGG47750.030.394995
ATGGGAG21500.029.8793325
CATGGGT34100.028.9560494
AGAGTAC242050.027.83035310-11
AGTACTT128700.026.59820712-13
Produced by FastQC (version 0.11.5)