FastQCFastQC Report
Fri 10 Feb 2017
ERR1147415.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147415.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3560648
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT396941.1147970818794781No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT236600.6644857902269475No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200320.5625942244220715No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67470.1894879808394427No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52870.14848420849238678No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA278000.048.783921
GGTATCA161150.045.218791
AACGCAG313000.043.1164556
ATCAACG354900.038.030343
TCAACGC357750.037.7563824
CAACGCA364550.037.084745
ACGCAGA364650.037.0256357
TATCAAC365700.036.9580842
CGCAGAG368050.036.6835988
GCAGAGT393700.034.535349
GTACATG312350.033.355261
TACATGG318600.032.563182
ACATGGG319150.032.0023
CATGGGT69300.030.5541114
CATGGGG168150.029.2524014
AGAGTAC384800.028.56404910-11
ATGGGGG62250.027.3262215
CAGAGTA390300.026.75193410-11
GAGTACT215900.026.4742512-13
TATCACG2256.5756467E-926.438912
Produced by FastQC (version 0.11.5)