FastQCFastQC Report
Fri 10 Feb 2017
ERR1147413.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147413.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3322320
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT281180.8463362951190734No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157190.47313323219918607No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147880.4451106455729731No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43770.13174528642635266No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39830.11988610368658045No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA187250.051.020441
GGTATCA105600.048.6449621
AACGCAG229400.041.5849086
TCAACGC251600.037.939294
ATCAACG252000.037.8830573
CAACGCA256100.037.3655365
TATCAAC259400.036.9656452
ACGCAGA267000.035.7510347
CGCAGAG271400.035.1275988
GCAGAGT292900.032.6912359
GTACATG230500.031.7632641
TACATGG238100.030.8789332
ACATGGG239050.030.2313883
CATGGGG131900.028.6772234
AGAGTAC283500.027.98514410-11
AGTACTT163850.025.93466212-13
CATGGGT49300.025.8161624
GAGTACT158900.025.65714812-13
ATGGGGG55550.025.3740085
CAGAGTA290600.025.28552210-11
Produced by FastQC (version 0.11.5)