FastQCFastQC Report
Fri 10 Feb 2017
ERR1147412.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147412.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1548944
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55490.358244068216798No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36230.23390129016930247No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30480.19677922507204906No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA46700.045.326521
GGTATCA29750.044.2941361
AACGCAG58750.035.9460266
GTACATG71200.035.5917931
TCAACGC60750.034.4688454
TACATGG74100.034.0963332
CAACGCA62000.033.8698585
ACATGGG75500.032.9351543
ATCAACG64100.032.8530433
CATGGGG44600.030.677744
ACGCAGA68900.030.5642977
TATCAAC69850.030.028682
ATGGGGG21950.029.269795
CGCAGAG72400.029.1689058
GCAGAGT79850.026.596459
TGGGGGG18000.025.7781936
CATGGGT16650.025.3673154
AGAGTAC73750.024.96485310-11
CAGAGTA76300.022.68813510-11
TATACTG4300.022.135125
Produced by FastQC (version 0.11.5)