FastQCFastQC Report
Fri 10 Feb 2017
ERR1147411.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147411.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2578671
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93170.3613101477466493No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51740.20064599167555688No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48480.18800382057269036No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA72450.052.9872251
GGTATCA39750.046.0389021
AACGCAG91450.042.0173236
TCAACGC93450.040.927124
CAACGCA96150.039.777845
ATCAACG96750.039.346723
ACGCAGA105950.036.4353757
GTACATG113450.034.8363571
CGCAGAG111650.034.4687168
TATCAAC111350.034.403272
TACATGG118100.033.1432042
ACATGGG117250.032.6195263
CATGGGT32450.031.5277714
GCAGAGT123100.031.4559239
AGAGTAC114550.027.2611210-11
CATGGGG64250.025.829184
GGGTTAG5600.025.5474131
ATGGGTA18050.025.3742395
CAGAGTA119250.023.76752910-11
GAGTACT58150.023.27079612-13
Produced by FastQC (version 0.11.5)