FastQCFastQC Report
Fri 10 Feb 2017
ERR1147410.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147410.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2460614
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111270.45220420594209415No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62210.2528230758664301No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59330.24111868013430796No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT30560.12419664360196277No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT25970.1055427629038931No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA24890.10115361450434729No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37050.063.7045821
GTATCAA76050.060.425311
TCAACGC105150.043.2232554
ATCAACG107400.042.373133
AACGCAG107600.042.1837926
CAACGCA108100.042.0437135
TATCAAC118900.038.57672
ACGCAGA124300.036.564167
CGCAGAG128650.035.3278278
GTACATG131750.034.78881
TACATGG135600.033.6939472
GCAGAGT137850.033.272169
ACATGGG136000.033.24363
CATGGGT46750.031.5575624
CATGGGG68150.028.6313194
AGAGTAC133600.027.6514910-11
ATGGGTG25950.026.5921845
GCGTATC1358.154004E-526.4899541
CAGAGTA137400.025.34974310-11
GTAAGTA10500.024.3946952
Produced by FastQC (version 0.11.5)