FastQCFastQC Report
Fri 10 Feb 2017
ERR1147409.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147409.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1641294
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67460.4110171608499148No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38810.23645976893841078No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36220.22067953699946505No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT18160.11064440618195155No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT16460.10028672498650455No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA54100.048.899991
GGTATCA31150.048.0107771
AACGCAG66900.039.566596
TCAACGC70400.037.768494
ATCAACG72050.036.9861223
CAACGCA71800.036.949215
GTACATG87550.033.4155351
ACGCAGA79550.033.2747347
TATCAAC82150.032.4774172
CGCAGAG81650.032.3460738
TACATGG90300.032.316132
GGGTTAG6500.032.0832021
ACATGGG89100.032.0448533
CATGGGT24350.031.5126954
GTATCGA1152.7700045E-531.0347449
ATGGGGG23400.030.5042365
GCAGAGT87400.030.2860989
CATGGGG47650.029.5855874
CGTATAC850.007221165627.9921253
GGTTAGA7400.026.557872
Produced by FastQC (version 0.11.5)