FastQCFastQC Report
Fri 10 Feb 2017
ERR1147408.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147408.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1648433
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66090.40092621295496994No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41720.25308884255532377No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35760.21693329361884894No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT16590.10064103302954988No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA54650.049.9677731
GGTATCA31100.049.462261
AACGCAG68450.039.626946
TCAACGC71700.037.6648144
ATCAACG72750.037.2029653
CAACGCA72950.037.019435
GTACATG83150.035.852751
ACGCAGA78700.034.4658747
TACATGG86750.034.267012
ACATGGG87350.033.7086563
CGCAGAG80950.033.581388
TATCAAC81800.033.1362422
TATAACG902.3764116E-433.095782
CATGGGT22650.033.0903634
GCAGAGT88200.030.8210039
CATGGGG48450.029.465694
ATGGGGG22500.027.4947625
AGAGTAC82400.027.14312210-11
TATACCG1401.0581412E-425.4930845
GAGTACT42650.024.89535712-13
Produced by FastQC (version 0.11.5)