FastQCFastQC Report
Fri 10 Feb 2017
ERR1147407.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147407.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2522634
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116730.462730622040296No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68540.2717001356518623No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61320.24307925763309302No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA80350.060.813171
GGTATCA39350.057.545131
AACGCAG108250.044.892236
TCAACGC108000.044.775854
ATCAACG109600.044.176463
CAACGCA110400.043.9102175
TATCAAC119900.040.6233982
ACGCAGA126000.038.6153347
CGCAGAG131050.037.218078
GCAGAGT142950.034.161439
GTACATG129050.032.9231381
TACATGG134150.031.8695472
ACATGGG134650.030.9220813
AGAGTAC135800.027.659910-11
CATGGGT39050.027.1129044
CATGGGG69850.025.8019944
TATAACG1859.205978E-725.7490022
CAGAGTA140450.025.43128610-11
GAGTACT70550.024.40779712-13
AGTACTT73500.024.19696612-13
Produced by FastQC (version 0.11.5)