FastQCFastQC Report
Fri 10 Feb 2017
ERR1147406.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147406.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2010771
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90840.4517670087742463No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53610.2666141494978792No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50600.2516447671067466No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA66600.053.239851
GGTATCA35500.051.199481
AACGCAG85050.041.7525836
TCAACGC88350.040.058424
CAACGCA90550.039.085165
ATCAACG90100.039.0823143
ACGCAGA99800.035.2837337
TATCAAC101000.035.2617152
CGCAGAG103150.034.080168
GTACATG104700.033.9229551
TACATGG108700.032.65432
ACATGGG108450.032.0307163
GCAGAGT113800.031.0475659
CATGGGT33500.027.5213814
TATCACG1306.340045E-527.4872672
AGAGTAC106850.027.13835110-11
CATGGGG56750.026.7274594
CAGAGTA109600.024.53077510-11
AGTACTT56300.024.3526312-13
GAGTACT53400.023.50306712-13
Produced by FastQC (version 0.11.5)