FastQCFastQC Report
Fri 10 Feb 2017
ERR1147405.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147405.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1315900
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57220.43483547382019916No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36080.27418496846264917No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30170.22927274108974846No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA45050.059.2543831
GGTATCA22600.057.475841
TCAACGC61350.042.953624
ATCAACG62000.042.599243
CAACGCA63900.041.2395065
AACGCAG64150.040.986066
TATCAAC66750.039.786892
ACGCAGA72150.036.523977
CGCAGAG74300.035.4670878
GCAGAGT81000.032.6802679
GTACATG77450.031.7735631
TACATGG79850.031.0223562
ACATGGG78650.030.2532353
CATGGGT24550.027.1379574
CATGGGG33350.027.11184
AGAGTAC77450.026.84332810-11
CAGAGTA78800.025.10013410-11
ACTAGAC2651.382432E-924.6920663
GTCTAAT2501.9790605E-823.8340171
AGTACTT38300.023.29716512-13
Produced by FastQC (version 0.11.5)