FastQCFastQC Report
Fri 10 Feb 2017
ERR1147404.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147404.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2766017
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131160.4741836366153932No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78890.28521155148359534No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75310.27226875322892086No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27930.1009755182271114No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46750.055.966281
GTATCAA91800.055.314691
AACGCAG122300.041.096936
TCAACGC124700.040.210574
ATCAACG127250.039.4515273
CAACGCA127650.039.32795
TATCAAC135900.037.256262
GTACATG143100.035.2351
ACGCAGA144100.034.9209067
CGCAGAG148500.033.8862158
TACATGG149200.033.8153882
ACATGGG148850.033.0472873
ATGGGGG47250.031.3456065
GCAGAGT163200.031.1619859
CATGGGG89400.030.9381684
AGAGTAC154850.027.54146610-11
TGGGGGG39250.026.9748786
AGTACTT84600.024.81895312-13
CAGAGTA157500.024.56664710-11
CATGGGT33150.024.223084
Produced by FastQC (version 0.11.5)