FastQCFastQC Report
Fri 10 Feb 2017
ERR1147403.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147403.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2482978
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94420.380269176770797No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58570.23588610128643914No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50820.20467358148159187No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT27360.11019026346588652No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA74400.049.3314441
GGTATCA42750.048.7806971
AACGCAG91650.039.845726
TCAACGC95500.038.0525324
CAACGCA97550.037.4967465
ATCAACG97500.037.271973
ACGCAGA107100.034.0976687
CGCAGAG110550.033.0873648
TATCAAC111800.032.864192
GTACATG108900.031.7880821
TACATGG113400.030.667572
GCAGAGT123350.029.6056759
ACATGGG112050.029.5657633
CATGGGG57250.026.6995164
AGAGTAC113750.025.80367310-11
CAGAGTA118450.024.22746710-11
CATGGGT31350.023.904454
GAGTACT58100.023.18664212-13
AGTACTT60900.022.75539612-13
GTACTTT67250.020.25298114-15
Produced by FastQC (version 0.11.5)