FastQCFastQC Report
Fri 10 Feb 2017
ERR1147402.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147402.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3269689
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161480.4938696004421215No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86860.26565217670549096No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86200.2636336361042289No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA121900.048.4577941
AACGCAG145500.040.4305426
GGTATCA71600.038.4611851
TCAACGC158650.037.116874
CAACGCA161400.036.484465
ATCAACG161650.036.3912353
GTACATG169500.035.306751
ACGCAGA168550.034.8661847
TATCAAC174400.034.0530742
TACATGG175500.033.805692
CGCAGAG174400.033.6625448
ACATGGG173450.033.641163
CATGGGT42550.031.173214
GCAGAGT190600.030.8638179
CATGGGG94200.028.666934
AGAGTAC179000.027.7299810-11
AGTACTT103050.024.18478812-13
ATGGGGG36000.023.4618425
GAGTACT96150.023.35306512-13
CAGAGTA185650.023.2444210-11
Produced by FastQC (version 0.11.5)