FastQCFastQC Report
Fri 10 Feb 2017
ERR1147401.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147401.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3038431
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136800.45023237322157394No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78100.25704055810383714No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74570.24542271981822195No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT30800.10136810742123155No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47350.057.290071
GTATCAA93800.056.8227961
TCAACGC128900.041.2076264
AACGCAG129600.040.939166
ATCAACG131650.040.3920333
CAACGCA132550.039.9831545
TATCAAC143950.037.4957662
GTACATG164850.037.250851
TACATGG169700.036.1242832
ACATGGG169450.035.3832743
ACGCAGA152250.034.9659047
CGCAGAG156400.034.1141628
CATGGGG84000.031.5816354
CATGGGT57750.031.5172654
GCAGAGT170150.031.427289
ATGGGGG43950.029.3683745
AGAGTAC163500.026.84835410-11
AGTACTT83000.024.15076412-13
ATGGGTG32150.024.0514585
CAGAGTA167950.023.97660610-11
Produced by FastQC (version 0.11.5)