FastQCFastQC Report
Fri 10 Feb 2017
ERR1147400.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147400.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2481266
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104090.4195035921178946No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59240.23874909018218926No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57090.23008415865126913No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA79650.050.818361
AACGCAG98250.040.987046
GGTATCA44000.040.2383731
TCAACGC102850.039.0960464
ATCAACG104950.038.370433
CAACGCA105200.038.2227065
ACGCAGA115300.034.874497
TATCAAC117500.034.573332
GTACATG122350.034.495781
CGCAGAG120050.033.4946148
TACATGG126300.033.3906332
ACATGGG124850.033.0644573
GCAGAGT130400.030.927349
CATGGGG65700.030.3298244
CATGGGT33350.026.9321824
AGAGTAC122450.026.30450610-11
GGGTTAG5550.024.704241
ATGGGGG31350.024.096655
CAGAGTA126900.022.96809210-11
AGTACTT63150.022.0881812-13
Produced by FastQC (version 0.11.5)