FastQCFastQC Report
Fri 10 Feb 2017
ERR1147399.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147399.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1843822
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77940.4227089165873929No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47280.25642388473507743No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44080.23906863026908237No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA56050.053.0612831
GGTATCA30600.052.9785731
AACGCAG71450.041.1215746
TCAACGC74200.039.597534
ATCAACG74950.039.1219333
CAACGCA75600.038.864245
GTACATG90450.035.5826261
TATCAAC83000.035.524842
TACATGG92950.034.6699262
ACGCAGA86350.034.2325367
ACATGGG93450.033.604673
CGCAGAG89300.033.1682788
CATGGGG53350.031.6613144
GCAGAGT98650.030.0848949
ATGGGGG29650.028.8856955
CATGGGT21650.028.2959564
TATACCG1704.442445E-727.9889165
AGAGTAC90900.026.23768410-11
GGGTTAG5050.024.7845331
CAGAGTA95850.023.8898610-11
Produced by FastQC (version 0.11.5)