FastQCFastQC Report
Fri 10 Feb 2017
ERR1147398.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147398.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1163918
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44890.3856800908655077No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28620.24589361106194768No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25580.21977493259834457No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA37400.046.683381
GGTATCA22300.045.4266051
AACGCAG47100.037.254876
TCAACGC48950.035.8468744
TATAACG1001.05696545E-535.725742
ATCAACG49300.035.471733
CAACGCA50600.034.7955065
ACGCAGA55450.031.6448087
TATCAAC56000.031.5789992
GTACATG53250.030.9974821
CGCAGAG57800.030.3582088
TACATGG54750.029.363622
ACATGGG54500.028.5947783
TAGTCCG850.00722135627.9912999
GCAGAGT62550.027.9577339
GTCTAGT1755.6265344E-727.2406961
CATGGGG29450.026.4586684
AGAGTAC59650.025.77698110-11
GTACTAA1802.1520551E-523.1735081
CTTAGCG1300.002055887622.8775023
Produced by FastQC (version 0.11.5)