FastQCFastQC Report
Fri 10 Feb 2017
ERR1147397.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147397.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2155996
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93060.431633453865406No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53650.24884090694045816No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50130.23251434603774773No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA74150.051.354671
AACGCAG89500.042.1298036
GGTATCA41250.040.883941
TCAACGC98200.038.3973244
CAACGCA99350.038.1923145
ATCAACG100350.037.5153923
ACGCAGA106000.035.740187
CGCAGAG109750.034.518998
TATCAAC111750.034.213322
GTATACG1051.4686146E-534.052751
GTACATG107800.032.5601771
GCAGAGT121550.031.3636259
ACATGGG108050.031.2641583
TACATGG111150.031.236822
CATGGGG58200.028.612664
AGAGTAC112950.027.82799510-11
AGTACTT62150.024.92813512-13
CATGGGT28600.024.3300514
GTATAGG3700.024.1590461
CAGAGTA116050.023.85600510-11
Produced by FastQC (version 0.11.5)