FastQCFastQC Report
Fri 10 Feb 2017
ERR1147396.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147396.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1875682
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83010.44255902653008344No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46250.24657697840038983No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45090.24039256121240168No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA66600.055.037411
GGTATCA34500.046.1263731
AACGCAG80550.045.043186
TCAACGC85250.042.4900934
CAACGCA87300.041.6285935
ATCAACG87250.041.4479373
ACGCAGA95350.038.176457
TATCAAC95950.038.119622
CGCAGAG98700.036.9409528
GTACATG103800.036.5188331
TACATGG107400.035.1649672
ACATGGG105600.034.97783
TATCACG1209.2079244E-734.7489742
GCAGAGT107950.033.7204679
CATGGGT29050.032.1453634
CATGGGG57150.030.390054
ATGGGGG29200.029.5358815
AGAGTAC103550.027.54249810-11
CTATGCG1358.2686E-526.435189
CCTATAC3250.025.621793
Produced by FastQC (version 0.11.5)