FastQCFastQC Report
Fri 10 Feb 2017
ERR1147395.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147395.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2126612
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93050.43755043233086244No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54900.2581571062328248No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51890.24400313738472276No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA83450.052.5117841
GGTATCA44800.044.715391
AACGCAG101450.043.09326
TCAACGC108400.040.110824
ATCAACG109900.039.563363
CAACGCA110800.039.510415
ACGCAGA115050.037.999187
CGCAGAG117600.037.0740558
TATCAAC118450.036.8183942
GTACATG123150.034.9541361
GCAGAGT129500.033.8509759
TACATGG126850.033.5818332
ACATGGG125000.033.261413
TATCACG1352.3079501E-630.8925022
CATGGGG56950.029.9751684
ATGGGGG27500.029.1994425
AGAGTAC123200.028.43662610-11
TATACCG2205.178663E-927.036525
CATGGGT24100.025.4210594
CAGAGTA127700.025.03577610-11
Produced by FastQC (version 0.11.5)