FastQCFastQC Report
Fri 10 Feb 2017
ERR1147393.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147393.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1572484
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35730.22722011797894287No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21080.13405541805194837No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18590.11822059874695068No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA41150.050.8197751
GGTATCA23500.047.4099161
AACGCAG52900.039.802526
TCAACGC54250.038.3734864
ATCAACG55100.037.7815173
CAACGCA55600.037.655715
ACGCAGA61200.034.598847
GTACATG74050.034.0338251
TATCAAC63050.033.1467482
ACATGGG75700.033.0001833
CGCAGAG64050.032.966458
TACATGG76050.032.8828122
GCAGAGT72950.029.2706349
CATGGGG42650.029.1467554
ATGGGGG19950.025.9381685
AGAGTAC67150.025.90853110-11
CATGGGT19150.024.2263934
CATATAC4300.023.5149153
CAGAGTA70400.023.10721410-11
GAGTACT28900.022.94774212-13
Produced by FastQC (version 0.11.5)