FastQCFastQC Report
Fri 10 Feb 2017
ERR1147392.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147392.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1032584
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41450.40142012659502757No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24870.24085207595701658No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20720.20066164108682683No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18350.058.422591
GTATCAA31700.052.9827081
AACGCAG39650.041.5479436
TCAACGC40900.040.568964
CAACGCA41800.039.6954655
ATCAACG42450.039.2277453
TATCAAC47700.035.1953742
GTACATG52650.034.3891
ACGCAGA48500.033.843897
TACATGG54350.032.860682
CGCAGAG50450.032.4178738
ACATGGG54450.032.1116333
ATGGGGG15000.029.736035
GCAGAGT55600.029.3081749
CATGGGT13800.029.3050734
CATGGGG30600.029.1529714
TATGTAA4150.025.821372
CAGAGTA54250.024.9947110-11
AGAGTAC52850.024.9816410-11
GAGTACT25950.023.37629912-13
Produced by FastQC (version 0.11.5)