FastQCFastQC Report
Fri 10 Feb 2017
ERR1147391.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147391.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences475876
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35150.7386377964007431No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20390.42847296354512515No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19260.4047272818969648No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6700.14079297968378315No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6510.13680034294648186No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC5050.10612008170195597No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27450.049.255921
GGTATCA15550.049.0291631
AACGCAG35750.036.928946
TATCAAC37900.035.49152
ATCAACG37900.035.4616663
TCAACGC38150.035.2292824
CAACGCA38900.034.24435
ACGCAGA41500.031.8122777
CGCAGAG41850.031.5462258
CGCATGT800.005372829.7344477
GTACATG38600.029.3184641
GCAGAGT45400.029.0795069
TACATGG39150.028.8853032
CATGGGG16950.028.4187644
ATGGGGG8250.028.112575
ACATGGG38150.028.0587223
GTATAGC1306.3249354E-527.4905131
GAGCTTA1306.39326E-527.4471846
TGCACTA900.00954776126.4306225
AGAGTAC44450.026.4231910-11
Produced by FastQC (version 0.11.5)