FastQCFastQC Report
Fri 10 Feb 2017
ERR1147390.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147390.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2260521
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96680.427689015054494No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56790.25122527063451305No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51560.22808901133853657No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA81250.048.178691
GGTATCA48900.042.5235521
AACGCAG94300.040.872956
TCAACGC103850.037.057024
ATCAACG105650.036.5382653
CAACGCA105850.036.3006445
ACGCAGA109750.035.010697
CGCAGAG114400.033.5876168
TATCAAC118050.033.3402982
GTACATG118700.032.8778651
TACATGG122850.031.8437522
ACATGGG121950.031.2643173
GCAGAGT125350.030.7959129
CATGGGT28800.028.294424
CATGGGG67600.027.7163814
AGAGTAC118100.026.11388810-11
CAGAGTA121450.024.36510310-11
ATGGGGG27650.023.4479055
GTAATAC3302.910383E-1123.4316083
TAAACGT2052.2712738E-623.21184
Produced by FastQC (version 0.11.5)