FastQCFastQC Report
Fri 10 Feb 2017
ERR1147389.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147389.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4310889
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145100.3365895062480152No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77700.18024124490331345No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76270.17692406369080718No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA130950.046.7306441
AACGCAG142500.042.198966
TCAACGC161750.037.434234
GTACATG184600.037.280961
CAACGCA164950.036.63595
ACGCAGA165150.036.375447
ATCAACG167500.036.1491783
GGTATCA74350.036.1436841
CGCAGAG168550.035.7828258
ACATGGG192400.034.747753
TACATGG199050.034.2812422
TATCAAC187450.032.4320532
GCAGAGT187950.032.2159469
CATGGGG104200.029.8537854
CATGGGT48950.028.919434
ATGGGGG48350.028.171145
AGAGTAC179050.027.65510710-11
TGGGGGG39050.023.9135516
CAGAGTA188700.023.05726810-11
GGGTTAG12450.022.9725251
Produced by FastQC (version 0.11.5)