FastQCFastQC Report
Fri 10 Feb 2017
ERR1147387.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147387.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3604904
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129520.35928834720702685No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73990.20524818414027116No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69880.19384704835413094No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA123550.047.03341
AACGCAG135950.042.0000846
ACGCAGA153650.037.1618047
TCAACGC156250.036.7337654
GTACATG164550.036.292291
CGCAGAG158400.036.047428
CAACGCA160100.035.924715
ATCAACG160150.035.839223
GGTATCA73500.034.6246221
TACATGG174800.034.07232
ACATGGG171150.034.057073
TATCAAC173050.033.3499372
GCAGAGT175050.032.720689
CATGGGT55200.030.9243774
CATGGGG83950.030.3236334
AGAGTAC168300.028.50220110-11
ATGGGGG36050.026.0681345
CAGAGTA173900.023.8220810-11
ATGGGTG36750.023.3057615
ATGGGTA25850.023.2390825
Produced by FastQC (version 0.11.5)