FastQCFastQC Report
Fri 10 Feb 2017
ERR1147386.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147386.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1890593
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82770.4377991455590918No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46250.2446322397258426No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45900.24278096872251193No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA65400.046.5855671
GGTATCA37900.042.7895741
AACGCAG74900.040.5027666
TCAACGC85700.035.5373844
GTACATG91050.034.96791
CAACGCA87900.034.6479385
ATCAACG87800.034.6196483
ACGCAGA88000.034.4733737
CGCAGAG90200.033.6985058
ACATGGG93700.033.582453
TACATGG94400.033.580192
TATCAAC95900.031.874422
GCAGAGT98250.031.1796329
CATGGGT25050.030.1572884
AGAGTAC94250.027.83258410-11
CATGGGG48200.027.0267224
ATGGGGG21350.026.4680545
ATGGGTG16700.025.289385
CAGAGTA97900.023.63541410-11
GAGTACT49800.023.5306112-13
Produced by FastQC (version 0.11.5)